Aloe+vera

toc Doesn't the above plant seem like the perfect solution for cooling down during a hot day in Singapore? //Aloe vera// gel is perhaps one of the most well known uses of the plant, used as sunburn remedies. The cooling and moisturising effect of //Aloe vera// makes it an important plant very widely used in many consumer products. However, there is more to this cute [|succulent herb] with fleshy leaves. Read on to find out more about this cool plant!
 * //Aloe vera// (L.) Burm. f. **

= 1. Description =

First, let us familiarise ourselves with the appearance of the //Aloe vera// plant. As a relatively common plant sometimes also grown within households, most Singaporeans are likely to have seen it before. //Aloe vera// is a stemless or very short-stemmed succulent herb. It typically grows from 0.3 to approximately 1 m tall, sometimes reaching 1.2 m, spreading by [|offsets][1]. The plant reaches maturity when it has a base of 8 cm or greater in diameter [2]. It matures differently depending on cultivating conditions.



The green to grey-green leaves are thick, fleshy and triangular, up to 0.9 m long, and are arranged in a rosette [1]. Some varieties show white flecks on their upper and lower stem surfaces [3]. The leaves are smooth while the margin of the leaf is serrated and has small white teeth. The most mature leaves are on the outer part of the rosette. Leaves are typically meaty if filled with gel that arises from the clear central [|mucilaginous] pulp [3].



The flowers are produced in summer on a [|spike inflorescence] up to 90 cm tall. The flowers are terminal and each flower is pendulous with a yellow [|tubular corolla] of 2–3 cm in length [1].



=1.1 Diagnosis = The species varies widely with regard to the white spots on the leaf [4]. There are some suggestions that //Aloe massawana//, which is also white-spotted, may be conspecific with the spotted form of //Aloe vera// and the descriptions are also similar [5, 6]. The differences lie in their distributions – //Aloe massawana// was described to occur near three East African ports (Massawa, Lumbo and Dar Es Salaam) while the white-spotted //Aloe vera// variation is found in Yemen and Saudi Arabia [7]. Considering these distributions, it has been suggested that //Aloe massawana// is an introduced species, possibly of Arabian origin, due to long-established trade links between East Africa and Arabia [7].

Other results by DNA comparison imply that //Aloe vera// is relatively closely related to //Aloe perryi// [8]. However, //Aloe perryi// is easily differentiated from //Aloe vera// by the teeth along the margin of the leaves, which are red in colour compared to the white in //Aloe vera//.



2. Biology

=2.1 Reproduction and Propagation=

The tubular and brightly-coloured features of the //Aloe vera// flowers, together with abundant nectar, point to ornithophily as the main method of pollination [9]. Sunbirds (Nectariniidae) are known to frequent aloe flowers in fields and in African gardens. They are also sometimes visited by bees [9].

=2.1.1 Inter-species hybridisation=

Most plants of the //Aloe// genus are self-incompatible, yet the flowers are protandrous. In other words, the flower anthers ripen and pollen is dispersed before the stigma is receptive, hence self-pollination would not occur anyway [10]. In an area where two or more species of //Aloe// flower at the same time, the main pollen vectors, sunbirds, fly indiscriminately from one species to another. Hybridisation is thus frequent [10]. It was reported by Reynolds [11, 12] that many natural hybrids occur, especially in South Africa, while Newton [14] reported a number in East Africa. In gardens and greenhouse collections, where there might be only one specimen of each species, almost all seeds produced are of hybrid origin [10]. In a tropical garden, hybrid seedlings can become established quickly, and numerous garden aloes that are difficult to identify are undoubtedly of this origin. Most of such aloes produce capsules, dry dehiscent fruits that split open at maturity to release seeds. As the inflorescences sway in the wind, the seeds, which are winged, are thrown out and blown away [10].

=2.2 Symbiotic relationships=

Similar to many other species in its genus, //Aloe vera// forms arbuscular mycorrhiza, a symbiosis with fungi that allows the plant better access to mineral nutrients in soil [14].

=3. Human associations=

=3.1 Cultivation=

Want to grow your very own //Aloe vera// plant? It's not that difficult! Although //Aloe vera// can be grown from its seeds, it is most easily propagated from suckers readily produced by the mother plant. The suckers are commonly referred to as “pups” [15]. Pups can be obtained by cutting it from the main root of the mother plant. Gently uproot the mother plant and find the point of attachment. (14) Pups can be safely removed when they have several sets of leaves. The young plants should be planted in their own pot and watered deeply [16].

Some pointers:


 * Aloe vera grows best under direct sun in well-drained, porous soil, but it also tolerates light shade. In Singapore, it grows well in pots that are sheltered from the rain. It is susceptible to fungal disease, so try to keep the leaves dry and keep soil from splashing on the leaves [1]. Refrain from over-watering to force the growth of new roots.
 * Check the soil prior to watering. Allow the soil to dry down to a depth of 7.5 to 10 cm (3-4 in) for older, well-established plants or 3.5-5 cm (1-2 in) for younger plants [17].
 * Multiple plants should be provided with individual pots or planted at least 60 cm apart outdoors [18].

Still feeling clueless? Watch the following video for more tips and information!

media type="youtube" key="e8SPPSSlsOI" width="560" height="315" align="center"

Save the pups! Original video from [|YouTube] by The Neals' Homestead =3.2 Economic importance=

Evidently, besides being cultivated as a 'household pet' for decorative purposes or simply to kill off boredom, //Aloe vera// is also propagated for agricultural, cosmetic, medicinal uses and more. Talk about many uses from just one plant!

Aloe vera is used in many consumer products including skin lotion, moisturizers, cosmetics, or ointments for minor burns and sunburns. The clear and slightly sweet-tasting tissue of the leaves are often chopped and added raw to beverages and desserts for its jelly-like consistence. The bitter, outer green layer of the leaf should be peeled away before consuming the inner flesh.



The same portion of the //Aloe vera// leaves can be made into a clear gel that is often applied to minor cuts and burns to promote healing. In folk medicine, it was even ingested as a cure for constipation [19]. However, there is little scientific evidence of the effectiveness or safety of //Aloe vera// extracts for either cosmetic or medicinal purposes. Instead, studies finding positive evidence are frequently contradicted by other studies [20-24]. Nevertheless, the plant extract continues to be widely used in a multitude of products and c osmetic and alternative medicine industries regularly make claims regarding the soothing, moisturizing, and healing properties of //Aloe vera// [20, 25].

Interestingly, aloe vera leaf extract that has not undergone the industrial decolorisation process is suggested to be actually unsafe! Its oral ingestion has been listed by the California Office of Environmental Health Hazard Assessment (OEHHA) to cause cancer or reproductive toxicity [26]. Although the topical use of aloe vera is not associated with significant side effects [27], oral ingestion of aloe vera may cause abdominal cramps and diarrhea, and may also decrease the absorption of drugs [27]. Meanwhile, studies by the International Agency for Research on Cancer (IARC) have found ingested non-decolorized liquid aloe vera [28] to be carcinogenic in animals, and state it as a possible carcinogen for humans as well [29]. Be careful what you eat!

= = =4. Distribution=

The full natural range of //Aloe vera// is unclear as the species has been widely cultivated around the world. Its native distribution is known to be from the northern parts of Africa [30]. It grows wild in tropical climates around the world and n <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">aturalised strands of the species occur in the southern half of the Arabian Peninsula, through North Africa (Morocco, Mauritania, Egypt), as well as Sudan and neighbouring countries, along with the Canary, Cape Verde, and Madeira Islands [31]. <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">The species was introduced to China and various parts of southern Europe in the 17th century [32]. The species is widely naturalized elsewhere, occurring in temperate and tropical regions of Australia, South America, Mexico, the Caribbean and southeastern US states [33]. Most of its distribution has been suggested to be a result of human cultivation [5], its introduced range including Hawaii, Pu erto Rico and the US Virgin Islands [34].



=5. Systematics and Taxonomy=

=5.1 Classification=


 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Domain **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: Eukaryota
 * **<span style="font-family: Arial,sans-serif;">Kingdom **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: Plantae
 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Phylum: **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Tracheophyta
 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Class: **<span style="font-family: Arial,sans-serif; font-size: 10pt;"> Liliopsida
 * **<span style="font-family: Arial,sans-serif; font-size: 10pt;">Order **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: Asparagales
 * **<span style="font-family: Arial,sans-serif;">Family **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: Asphodelaceae (previously known as <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Xanthorrhoeaceae ) [35]
 * **<span style="font-family: Arial,sans-serif;">Genus **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: //Aloe//
 * **<span style="font-family: Arial,sans-serif;">Species **<span style="font-family: Arial,sans-serif; font-size: 10pt;">: //Aloe vera// (L.) Burm. f.

=5.2 Synonymy= <span style="font-family: Arial,sans-serif;">Vernacular names: Barbados Aloe, Medicinal Aloe, Burn Plant, Aloe, Chinese Aloe, Indian Aloe, True Aloe, Burn Aloe, First Aid Plant [1, 36] = = =5.3 Original genus description=
 * **Synonyms** || **Author** ||
 * //<span style="font-family: Arial,sans-serif;">Aloe barbadensis // || Mill. ||
 * //<span style="font-family: Arial,sans-serif;">Aloe barbadensis //<span style="font-family: Arial,sans-serif;">var. //<span style="font-family: Arial,sans-serif;">chinensis // || Haw. ||
 * //<span style="font-family: Arial,sans-serif;">Aloe chinensis // || (Haw.) Baker ||
 * //<span style="font-family: Arial,sans-serif;">Aloe elongata // || Murray ||
 * //<span style="font-family: Arial,sans-serif;">Aloe flava // || Pers. ||
 * //<span style="font-family: Arial,sans-serif;">Aloe indica // || Royle ||
 * //<span style="font-family: Arial,sans-serif;">Aloe lanzae // || Tod. ||
 * //Aloe perfoliata// var. //vera// || L. ||
 * Aloe rubescens || DC. ||
 * //<span style="font-family: Arial,sans-serif;">Aloe vera //<span style="font-family: Arial,sans-serif;">var. //<span style="font-family: Arial,sans-serif;">chinensis // || (Haw.) A. Berger ||
 * //Aloe vera// var. //lanzae// || Baker ||
 * //Aloe vera// var. //littoralis// || J.Koenig ex Baker ||
 * //<span style="font-family: Arial,sans-serif;">Aloe vulgaris // || Lam. ||
 * //<span style="font-family: Arial,sans-serif;">Aloe maculata // || Forssk. (illegitimate) ||
 * //<span style="font-family: Arial,sans-serif;">Aloe variegata // || Forssk. (illegitimate) ||
 * //<span style="font-family: Arial,sans-serif;">Aloe vera // || Mill. (illegitimate) ||

=5.4 Etymology=

<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">This species was originally described as //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">Aloe perfoliata //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;"> var. //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">vera //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;"> in 1753 by Carl Linnaeus [38]. In 1768, it was then described as //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;"> Aloe vera //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">by Nicolaas Laurens Burman in //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">Flora Indica //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">, vol 83. Just about ten days later, it was again described by Philip Miller in the //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">Gardener’s Dictionary //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">as //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">Aloe barbadensis //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">, another commonly known synonym of the species [39]. <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10.5pt;">The genus name arises from the Arabic word ‘alloeh’, meaning bitter and shining. The bitter leaf exudate, which produces the cathartic effects which the plant is widely used for, is derived from the peripheral bundle sheath cells of the leaves [19]. ‘Vera’, meanwhile, means true or genuine [34]. =5.5 Type Information=

<span style="font-family: Arial,sans-serif; font-size: 10pt;">The type specimen NHMUK BOT BM000958260, identified by P. Rupele and collected from India, is currently stored in the Natural History Museum, Cromwell Rd, Kensington, London SW7 5BD, UK, under the Natural History Museum (London) Collection Specimens dataset [40]. It is not specified if it is the holotype and is likely to be a paratype.



=6. Phylogeny=

=6.1 Phylogenetic position in the Asparagales=

The order Asparagales can be divided into a basal paraphyletic group, the "lower Asparagales". This includes the Asphodelaceae as defined here [41] and a well-supported monophyletic group of "core Asparagales", comprising Amaryllidaceae //sensu lato// and Asparagaceae //sensu lato// [42]. Three separate families were at one time recognized (e.g. in the first APG system of 1998): Asphodelaceae, Hemerocallidaceae and Xanthorrhoeaceae. Molecular phylogenetic studies have shown that the three are closely related [43, 44], although Rudall [45] considered that the combination into a single clade was not supported by morphological analysis. The most recent APG classification, the APG IV system of 2016, places the three former families into a single family, the Asphodelaceae //sensu lato//. The former families are treated as three subfamilies: Asphodeloideae, Hemerocallidoideae and Xanthorrhoeoideae [46].

The following phylogenetic tree for Asphodelaceae //sensu lato// is based on a molecular phylogenetic analysis of the DNA sequences of the chloroplast genes rbcL, matK, and ndhF [47]. All branches have at least 70% bootstrap support. Of the 36 genera recognized by the authors, 29 were sampled.



Aloes are now placed in the family Asphodelaceae. They had been considered part of the lily family (Liliaceae) for years under the Cronquist system of 1981 [48, 49]. This was because of the difficulty Cronquist faced in <span style="color: #222222; font-family: Arial,sans-serif; font-size: 14px;">classifying the less obviously delineated lilioid monocots, and placed taxa from both modern orders Asparagales and Liliales into a single family [45]. Although still related to the lily, aloes and other plants now in the Asphodelaceae family have been found to have significant morphological and ecological differences from the lilies. Both the Liliaceae and Asphodelaceae, along with about 20 other families, are part of the larger order of plants known as Asparagales, which distinguishing characteristic includes their pigment rich black seeds [11].

=6.2 Phylogenetic position in the //Aloe// genus=

<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">All //Aloe// taxa ( <span style="color: #403838; font-family: 'Cambria Math',serif; font-size: 9.5pt;">∼ <span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">400 species) share a conserved bimodal karyotype with a basic genome of four large and three small submetacentric/acrocentric chromosomes. In a study by Adams et al. [50], the physical organization of 18S–5.8S–26S and 5S ribosomal DNA (rDNA) in <span style="color: #403838; font-family: Arial,sans-serif; font-size: 12.6667px;"> 13 //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 12.6667px;">Aloe //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 12.6667px;"> species was investigated <span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">using fluorescent in situ hybridization (FISH). The organization was compared with a phylogenetic tree of 28 species (including the 13 used for FISH) constructed by sequence analysis of the internal transcribed spacer (ITS) of 18S–5.8S–26S rDNA. The phylogeny showed little divergence within //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">Aloe, //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;"> although distinct, well-supported clades were found. FISH analysis of 5S rDNA distribution showed a similar interstitial location on a large chromosome in all species examined. In contrast, the distribution of 18S–5.8S–26S rDNA was variable, with differences in number, location, and size of loci found between species. Nevertheless, within well-supported clades, all species had the same organizational patterns. Thus, despite the striking stability of karyotype structure and location of 5S rDNA, the distribution of 18S–5.8S–26S rDNA is not so constrained and has clearly changed during //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">Aloe //<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;"> speciation.



<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;"> The tree shows there is little divergence within //Aloe// as a whole, although several consistent clades were detected. The clade containing //Aloe tenuior// and //Aloe ciliaris// was sister to the rest. Both these species were used for FISH analysis. The tree also showed a central clade (from //A. vera// to //A. inermis//) with high bootstrap support of 96.

<span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">Quoting the conclusions of the paper, with the exceptions of //Aloe ngobitensis// and //Aloe nyeriensis//, the locations of 18S–5.8S–26S rDNA appear to be consistent with the ITS tree; species within the same clade of the ITS phylogeny had the same number and distribution of loci. This number of shared rDNA locations and clade memberships is unlikely to be due to chance. The patterns seem too congruent to be an artifact, and there are two possible explanations for this:
 * 1) The ITS sequence data reflect the history of rDNA chromosome distributions in //Aloe// arising by amplifications and reductions of different rDNA units at individual loci rather than a phylogeny of species relationships.
 * 2) The ITS sequence data do reflect a phylogeny of evolutionary relationships, and related species have similar distributions and sequence composition of 18S–5.8S–26S rDNA.

These seem to give some evidence of DNA phylogeny despite Reynolds [11, 12] <span style="color: #403838; font-family: Arial,sans-serif; font-size: 9.5pt;">classifying //Aloe// using morphological and floral features [50].

=7. Useful links=


 * 1) // Aloe vera // in [|Barcode of Life Data Systems (BOLD).]
 * 2) // Aloe vera // in Encyclopedia of Lfei
 * 3) //Aloe vera// in [|GBIF]
 * 4) // Aloe vera // in [|Catalogue of Life]
 * 5) // Aloe vera // in [|Open Tree of Life]
 * 6) // Aloe vera // in [|Biodiversity Heritage Library]
 * 7) // Aloe vera // in GenBank Database
 * 8) // Aloe vera // in [|Flora Fauna Web]



=8. References=


 * Page done by: ** Tay Siang Lin (a0131343@u.nus.edu)

**Literature cited:**


 * 1) <span style="font-family: Arial,sans-serif; font-size: 10pt;">National Parks Board. (2013). //Aloe vera (L.) Burm.f.// Retrieved from https://florafaunaweb.nparks.gov.sg/Special-Pages/plant-detail.aspx?id=1643
 * 2) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Yates, A., & LTD, C. (1973). //Yates Garden Guide//.
 * 3) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">McLeod, J. (2002). Australia Botanic Pocket Gardening Encyclopedia for Australian Gardeners.
 * 4) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Sharma, G. P., Rani, A., Zala, D. A., Sain, M., Singh, A., & Rathore, S. (2013). //Aloe barbadensis// Miller a valuable ingredient for traditional uses and toxicological properties-A Review. //Int. J. Rec. Biotechnol//, //1//, 48-54.
 * 5) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Lyons, G. (2008). //The Definitive Aloe vera, vera//. Huntington Botanic Gardens.
 * 6) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Akinyele, B. O., & Odiyi, A. C. (2007). Comparative study of the vegetative morphology and the existing taxonomic status of //Aloe vera// L. //Journal of plant Sciences//, //2//(5), 558-563.
 * 7) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Wood, J. R. I. (1983). The Aloes of the Yemen Arab Republic. //Kew bulletin//, 13-31.
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 * 9) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Reynolds, T. (2004). //Aloes: the genus Aloe//. CRC press.
 * 10) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Newton, L. E. (2004). //Aloes in habitat// (pp. 3-36). CRC Press: Boca Raton.
 * 11) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Reynolds, G. W. (1982). //The Aloes of South Africa//. Rotterdam: AA Balkema.
 * 12) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Reynolds, G. W. (1966). Aloes of tropical Africa and Madagascar.
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 * 14) <span style="font-family: Arial,sans-serif; font-size: 10pt;">G <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">ong, M., Wang, F., & Chen, Y. (2002). Study on application of arbuscular-mycorrhizas in growing seedings of Aloe vera. //Zhong yao cai Journal of Chinese medicinal materials//, //25//(1), 1-3.
 * 15) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Eshun, K., & He, Q. (2004). //Aloe vera//: a valuable ingredient for the food, pharmaceutical and cosmetic industries—a review. //Critical reviews in food science and nutrition//, //44//(2), 91-96.
 * 16) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Das, P., & Panda, P. C. (1995). Protected cultivation of cacti and other succulents. //Advances in Horticulture//, //12//(2), 819-851.
 * 17) <span style="font-family: Arial,sans-serif; font-size: 10pt;">Das, N., & Chattopadhay, R. N. (2004). //Commercial Cultivation of Aloe//. Retrieved from http://nopr.niscair.res.in/bitstream/123456789/9406/1/NPR%203(2)%2085-87.pdf
 * 18) <span style="font-family: Arial,sans-serif; font-size: 10pt;">UC Davis Botanical Conservatory. (2009). //The Genus Aloe//. Retrieved from http://greenhouse.ucdavis.edu/files/botnot_01-01.00.pdf
 * 19) <span style="color: #333333; font-family: Arial,sans-serif; font-size: 10pt;">Shelton, R. M. (1991). //Aloe Vera//. //International Journal of Dermatology//, 30: 679–683. doi:10.1111/j.1365-4362.1991.tb02607.x
 * 20) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Boudreau, M. D., & Beland, F. A. (2006). An evaluation of the biological and toxicological properties of //Aloe barbadensis// (miller), //Aloe vera//. //Journal of Environmental Science and Health Part C//, //24//(1), 103-154.
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 * 22) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Marshall, J. M. (1990). //Aloe vera// gel: what is the evidence. //Pharm. J//, //244//, 360-362.
 * 23) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Vogler, B. K., & Ernst, E. (1999). //Aloe vera//: a systematic review of its clinical effectiveness. //Br J Gen Pract//, //49//(447), 823-828.
 * 24) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">Feily, A., & Namazi, M. R. (2009). //Aloe vera// in dermatology: a brief review. //Giornale italiano di dermatologia e venereologia: organo ufficiale, Societa italiana di dermatologia e sifilografia//, //144//(1), 85-91.
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 * 26) <span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">U.S. Office of Environmental Health Hazard Assessment. (2015). //<span style="font-family: Arial,sans-serif; font-size: 10pt;">Proposition 65. Chemicals Listed Effective December 4, 2015 as Known to the State of California to Cause Cancer: //Aloe Vera//, Non-Decolorized Whole Leaf Extract and Goldenseal Root Powder<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;">. //<span style="color: #222222; font-family: Arial,sans-serif; font-size: 10pt;"> Retrieved from https://oehha.ca.gov/proposition-65/crnr/chemicals-listed-effective-december-4-2015-known-state-california-cause-cancer
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